Journal: Journal of biomechanics
Article Title: Mesenchymal stem cells differentiate dynamic from static load through variable regulation of the Hippo pathway
doi: 10.1016/j.jbiomech.2026.113228
Figure Lengend Snippet: ( A ) Cell lysates from SS treated cells were immunoprecipitated for MST1/2 and immunoblotted for PP2Ac. Representative densitometry shows increased association between MST1/2 and PP2Ac, (n = 3, p < 0.05). ( B ) Pulling down lysates with AB to PP2Ac before MST1/2 IB confirmed this result. ( C ) DS reduced the association between PP2AC and MST1/2. ( D ) Cells were stained with anti-PP2Ac (red) showing increase in nuclear PP2Ac signal after SS, scale bar = 25 μm. Fluorescence intensity of nucleus/cytoplasm of PP2Ac confirmed a significant effect of SS, **** = p < 0.0001. ( E ) MST1/2 (green) showed unchanged cellular localization after strains.
Article Snippet: AMOT 130 (cat#: 50–173-1230, Fisher), YAP (D8H1X) (cat#: 14074S, Cell Signaling), Phospho-YAP-ser127 (cat# 13008S, Cell Signaling), PP2A-Cα/β (cat# sc-80665, Santa Cruz), LATS1 (cat# 3477S, Cell Signaling), Phospho-LATS1 (Ser909) (cat# 9157S, Cell Signaling), MST1 (D8B9Q) (cat#14946S, Cell Signaling), Phospho-MST1/2 (Thr183) (cat# PA540255 , Invitrogen), Phosphoserine antibody (cat# AB9332, Abcam), Tubulin (cat# sc-23949, Santa Cruz).
Techniques: Immunoprecipitation, Staining, Fluorescence